Protein expression systems have been used for decades, especially for research purposes. However, their uses have widened in recent years, and they are increasingly applied in areas such as gene editing and drug and pharmaceutical manufacturing processes. The future indicates positive prospects and heavy reliance on these systems to meet the changing research needs and protein application processes.
As the usage increases, developers create new systems to develop different proteins. The systems are other due to the processes involved and the resources needed to achieve the desired goals. Due to the varying needs, one should be able to customize the protein expression system to meet their needs. If you do not know about customizing the system, you can purchase or utilize either of these five systems.
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The Hypertrans custom protein system
The Hypertrans protein production system uses natural products to develop quality proteins through a transient plant expression system. This process is more efficient than other production systems since it can produce quality plant materials daily. Despite being slow, the method produces extremely high-level proteins without the viral replication used in other processes.
The system incorporates a proprietary modular binary vector to develop sequences and introduce expression cassettes and polypeptides to produce high-quality protein products. These new technologies and vectors enable the system to engage in direct gene cloning while restricting enzyme-based cloning. The vectors make the production system quick and easy to reach maximum yields.
This system is currently suitable for producing complex proteins such as enzymes and antibodies needed for research purposes. The system can also have antigens used for vaccine development through various processes such as nanotechnology. The system also produces metabolites needed for metabolic pathway engineering in biochemical processes that can sometimes be rare and difficult to synthesize through the ordinary chemical synthesis process.
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Stable Cell Line Generation Services
This Custom Protein Synthesis process produces quality production by generating the most stable cells in the market. The process promotes the gene sub-cloning process to produce high-performance expression vectors and increases the gene synthesis to optimize the expressed proteins. The process has host cells generated through the complex gene DNA editing and transference system. The process utilizes the selection of high-quality cell clones.
The system incorporates improving stable cell lines using various enzymes such as glutamine synthetase (GS) and dihydrofolate reductase (DHFR) to produce vital metabolites and disrupt the metabolic pathways to produce target metabolites.
The process also utilizes various inhibitor concentrations to promote gene amplification to boost the potency of assembled genes and protein products. The method also uses highly productive and stable CHO cells to deal with or eliminate protein expression and evaluation challenges.
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Recombinant Protein Expression Systems
This protein production system involves the transfer of cells containing some desired genes in a DNA vector. The genes are then subjected to cellular machinery, cell lysing, and purification steps to produce the final protein product. The process also uses the Prokaryotic and Eukaryotic expression systems to produce the top-quality protein products.
Due to the complex and advanced processes applied, this process can produce the desired protein within the shortest duration. The process can also incur lower costs to produce the desired protein products; however, it can be difficult for the system to express some mammalian proteins. The process can sometimes face various challenges, such as the degradation of expressed proteins caused by the host protein contamination.
The process uses other protein expression processes, such as the yeast expression system, which uses the economic eukaryotic expression system to produce the highly demanded intracellular proteins. The mammalian cell expression system enables the system to produce complex mammalian proteins which can be used to modify genetic cell structures within the body of a mammal. Finally, the insect cell expression system is ideal for inserting desired genes into insect cells.
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ExpiCHO Expression System
This process eliminates various uncertainties expressed in other protein development processes. The process optimizes the protein production process to produce highly-adaptable cells to produce a protein that meets qualities such as serum-free suspension and high-density mammalian and plant proteins. The process utilizes various nanotechnology procedures to produce twenty percent more proteins in fewer volumes. The system produces high protein yields using multiple protocols to boost protein production.
The systems use a seven-step protein production process and customize every stage to boost the final yield. You can also replicate and customize the process based on the resources and time and still end up with the desired protein genes; however, the quality will vary.
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Magic Cell-free Membrane Protein Expression
This process guarantees cost-friendly yet high-quality proteins within the shortest durations. The production system addresses challenges imposed by cultural barriers to the adoption of cell-free membrane protein expression by developing an advanced eukaryotic cell-free expression system that produces higher yields in batch mode.
The production kit uses the tobacco cells’ lysates which contain in vitro transcription and translation reactions. It utilizes various vectors such as vectors 1 and 2 to produce high yield proteins within 48 hours. The process is convenient, making it suitable for different protein expressions, and can be applied to large-scale processes such as targeting membrane proteins. The process can also be applied in over 20 areas such as expression analysis, metabolic, herbicide screening, and protein optimization. The process can help researchers resolve challenges such as obtaining membrane proteins that can be difficult to produce within the shortest duration.
Conclusion
There are various protein expression systems available in the market that one can adopt. You can also customize these processes to meet your exact needs, such as research, gene editing, etc. The selection process should be based on various factors such as the anticipated yield, duration of production, the cost to produce the protein, and the system’s flexibility.
